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Epithelial‐like cells in culture derived from human gingiva: response to nickel
Author(s) -
JACOBSEN NILS
Publication year - 1977
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.1977.tb02116.x
Subject(s) - connective tissue , fibroblast , chemistry , epithelium , fetal bovine serum , resorption , trypsin , tissue culture , fetus , cytotoxicity , cell growth , cell culture , andrology , toxicity , cell , endocrinology , biology , pathology , medicine , biochemistry , in vitro , pregnancy , enzyme , genetics , organic chemistry
— To obtain gingival cell cultures, human gingival tissue was minced and/or subjected to trypsin treatment with or without prior separation into epithelial and connective tissue portions. The tissues were men incubated in Eagle/Earle's MEM with 10% fetal calf serum in a humidified atmosphere of 5% CO 2 in air. Fibroblast‐like cell cultures were regularly obtained, and one culture showed epithelial‐like cell islets that could be transferred and kept in continuous culture. These epithelial‐like cells exhibited bone resorption stimulating activity as seen in gingival tissue and retained their growth pattern after prolonged storage. They were able to grow at serum concentrations down to 2.5% and with equal doubling time (about 17 h) in rich or minimum essential media. Exposure to nickel gave toxic effects on the growth at concentrations down to 2.5 μg/ml of nickel. In spite of the affinity of nickel to certain serum components, the serum concentration did not appear to be of specific importance to protect or aggravate the toxicity. It is felt that these cells may be of value for research on cytotoxicity of dental materials.