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On the mechanism of cytotoxicity of silver and copper amalgams in a cell culture system
Author(s) -
LEIRSKAR JAKOB
Publication year - 1974
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.1974.tb01902.x
Subject(s) - incubation , copper , mercury (programming language) , cadmium , zinc , toxicity , chemistry , cytotoxicity , nuclear chemistry , biochemistry , in vitro , organic chemistry , computer science , programming language
– Silver and copper amalgams show a pronounced cytotoxic effect on monolayer cultures of human epithelial cells (NCTG 2544). Analyses of the medium from silver amalgam cultures showed that, zinc was released in substantial amounts (14 μg/ml after incubation for 24 h). Small amounts of mercury and possibly also some silver were released, whereas release of copper and tin could not be. detected. Toxicity tests showed that 10 μg Zn 2 +/ml reduced the number of cells by 96% after incubation for 24 h. In the copper amalgam cultures about. 100 μ g copper and 5 μg, cadmium per ml medium were found after 24 h. Only small amounts of mercury were released, Toxicity tests showed an increasing effect of Cu 2 + and Cd 2 + with tune. With 50 μg Cu 2 +/ml the number of cells was reduced by 73% after incubation for 24 h, and after 3 d a similar effect was found with 25 μg/ml. With 10 μg Cd 2 +/ml no cells were left after 24 h, whereas after 3 d 1μg/ml reduced cell growth by more than 80 %.