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The acquired pellicle: enzyme and antibody activities
Author(s) -
öRSTAVIK DAG,
KRAUS FREDERICK W.
Publication year - 1974
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.1974.tb00381.x
Subject(s) - lysozyme , in vivo , antibody , in vitro , enzyme , chemistry , enzyme assay , biochemistry , amylase , saliva , specific activity , biology , immunology , microbiology and biotechnology
— Assays for enzyme and antibody activities have been applied to evaluate functional properties of proteins in 2‐h, experimental acquired pellicles. Enzyme activity was demonstrated for human salivary lysozyme and amylase in pellicles grown in vivo as well as in vitro , and antibody activity was demonstrated for rabbit immunoglobulins incorporated in pellicles grown in vitro . Desorption of pellicles prior to functional tests variously affected the activity of the proteins under study, thereby implying differences in the tenacity of protein binding to the pellicles.