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Activities of glycolytic and citric acid cycle enzymes in oral leukoplakia
Author(s) -
HAMMAR HANS,
ALVARES OLAV
Publication year - 1974
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.1974.tb00370.x
Subject(s) - leukoplakia , nad+ kinase , isocitrate dehydrogenase , dehydrogenase , lesion , enzyme , buccal mucosa , lactate dehydrogenase , biology , oral mucosa , chemistry , biochemistry , medicine , pathology , oral cavity , anatomy , dentistry , cancer
— Biopsies of homogeneous leukoplakias and non‐involved oral mucosa were obtained from the same patient, and buccal mucosa samples were obtained from individuals free of oral mucous membrane disease. Activities of glucose‐6‐phosphate dehydrogenase (G6PDH), pyruvate kinase (PK), glycerol‐3‐phosphate dehydrogenase (G3PDH), NAD and NADP dependent isocitrate dehydrogenases (ICDH) were quantitated in microdissected specimens of the superficial third of the epithelium. ICDH (NAD) was measured photo‐kinetically with the aid of a bioluminescent system. The remaining enzymes were assayed according to the techniques developed by l owry and co‐workers. The activities of G6PDH and G3PDH in non‐involved mucosa of leukoplakia patients were double those of mucosa from healthy controls. The activities of PK and ICDH (NAD and NADP) in these two groups of lesion‐free mucosa were similar. Non‐involved mucosa differed from leukoplakias with respect to the two ICDH's. In hyperorthokeratinized leukoplakias, NAD and NADP dependent ICDH activities were decreased by 59% and 37%, respectively. The values for the two ICDH's in hyperparakeratinized lesions lay between the levels of activity in noninvolved mucosa and hyperorthokeratotic leukoplakias but did not differ significantly from either.