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A method for isolation of anaerobic bacteria from endodontic specimens
Author(s) -
BERG JANOLOF,
NORD CARLERIK
Publication year - 1973
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.1973.tb01507.x
Subject(s) - isolation (microbiology) , bacteria , anaerobic bacteria , anaerobic exercise , microbiology and biotechnology , oxygen , chemistry , biology , chromatography , physiology , genetics , organic chemistry
– A method for the isolation of anaerobic bacteria from endodontic specimens under continuous anaerobiosis is described. A mobile anaerobe unit supplies a gentle stream of oxygen‐free gas over the tooth when the bacteriologic sample is taken. The paper‐points are then, under oxygen‐free gas, immediately transferred to prereduced, anaerobically sterilized roll tubes. The different isolated anaerobic bacteria are typed with biochemical tests, antibiotic susceptibility testing and gaschromatographic analysis of volatile fatty acids and alcohols.