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Exudation and leukocyte emigration in progesterone and estrogen treated rats
Author(s) -
LINDHE J.,
HELLDÉN L.,
LUNDGREN D.
Publication year - 1972
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.1972.tb00309.x
Subject(s) - estrogen , saline , granulation tissue , hormone , exudate , endocrinology , medicine , chemistry , intraperitoneal injection , wound healing , pathology , surgery
– The present investigation was performed in order to study the influence of an altered level of female sex hormones on the inflammatory reaction provoked by factors in human plaque. Forty female rats were oophorectomized and divided into four groups of 10 rats each. The animals were given daily intramuscular injections of progesterone (Group 1), estrogen (Group 2), progesterone combined with estrogen (Group 3), and oleum arachidis (Group 4) for a period of 19 days. On Day 14 perforated cylinders of Teflon® were installed subcutaneously in the back of the animals. Five days after implantation the wound fluid collected in the chambers was sampled (aspiration A). Four hundred μl of a plaque filtrate and saline was injected into the chambers. Five hours later the fluid within the chambers was aspirated anew (aspiration B). The volume of wound fluid and numbers of leukocytes/μl.d of wound fluid were assessed. The results show that oophorectomized rats with an increased progesterone level develop more pronounced signs of acute inflammation than control animals. The injections of plaque filtrate into the wound chambers initiated a more pronounced inflammatory reaction than saline injections. The plaque filtrates not only induced an increased exudation from the vessels of the granulation tissue surrounding the perforated chamber walls, but also stimulated the emigration of leukocytes. The effect of the plaque filtrate on developing acute inflammation was more marked in progesterone‐treated animals than in controls.