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Incisional wounds in palatal epithelium in guinea pigs
Author(s) -
FEJERSKOV O.,
PHILIPSEN H. P.
Publication year - 1972
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.1972.tb00262.x
Subject(s) - stratum spinosum , stratum granulosum , epithelium , stratum corneum , stratified squamous epithelium , pathology , anatomy , basal (medicine) , wound healing , biology , medicine , surgery , insulin
– Standardized incisional wounds were made in the palatal mucosa of 54 adult, male guinea pigs, in order to study the reaction to injury of orthokeratinized epithelium. The wound area was studied histologically 2, 4, 6, 12, 24, 48, 72, 120, and 240 hours postoperatively. Initial cellular changes occurred as early as 2 hours after wounding. The changes consisted of a reduced cytoplasmic stainability with pyknosis and karyorrhexis covering a triangular zone (reactive zone) along the incisional cleft involving the lower part of stratum corneum, stratum granulosum, and the upper half of stratum spinosum. It is suggested that this cellular reaction represents an “induced keratinization”. After 6 hours the reactive zone was delineated from the adjacent, normal epithelial cells by a demarcation zone consisting of 2–3 layers of hyperchromatic, elongated cells. The first signs of migratory activity were noticed from the basal and lower spinous cells 12 hours after wounding. Eighteen hours postoperatively a fusion of the horizontally migrating epithelium had occurred in all wounds examined. By 18 and 24 hours the demarcation zones of the two wound margins met and formed a continuous zone at the base of the incision to constitute the superficial cell layers of the fusion area. A gradual increase in number of cell layers was seen in the fusion area from 24 hours onwards, reaching normal height and differentiation of the epithelium at 120 hours. During the same period epithelial cells migrated from the basal cells into the fibrin‐filled connective tissue wound. By 240 hours the migratory activity was reduced to focal single cell migration. Although a normal cellular differentiation was present in the epithelium of the wound area at 240 hours, the characteristic histo‐architectural pattern of the area had not yet been re‐established, and no PAS‐positive basement membrane was found.