Premium
Studies on long‐term maintenance of teeth and viable associated cells in vitro
Author(s) -
LITWIN J.,
LUNDQUIST G.,
SÖDER P.Ö.
Publication year - 1971
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.1971.tb02052.x
Subject(s) - trypsinization , in vitro , dentistry , agar , agar plate , chemistry , biology , medicine , biochemistry , bacteria , trypsin , genetics , enzyme
– The present study describes techniques for maintaining teeth in vitro in which the cells surrounding the root are viable and able to multiply. Microbial contamination could be controlled by use of antibiotics. The material consisted of 20 extracted human teeth. Immediately after extraction the teeth were put in Eagle's medium and incubated at 37°C. Cells surrounding the root could be kept viable in a liquid medium or fixed in a semi‐solid medium containing Eagle's + 1 % agar. Viable cells could be trypsinized from teeth after as long as one year in vitro and grown in glass culture bottles.