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Preparation of human premolar roots for scanning electron microscopy
Author(s) -
VAM EINAR K.
Publication year - 1971
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.1971.tb02015.x
Subject(s) - collagen fiber , premolar , scanning electron microscope , distilled water , formaldehyde , fixation (population genetics) , fiber , chemistry , materials science , dentistry , anatomy , chromatography , composite material , biology , medicine , molar , biochemistry , gene
– The influence of various technical procedures on the morphologic details of human premolar root surfaces was investigated. Sixteen formaldehyde‐fixed specimens, in some cases post‐fixed with OsO 4 , were air‐dried at room temperature or freeze‐dried. Four of eight air‐dried specimens were fixed in formaldehyde only; another four were also post‐fixed with OsO 4 . Four freeze‐dried specimens were dried from distilled water and four from amylacetate. There was no noticeable difference between the specimens with regard to the gross and fine structure of fibrous collagen tissue. The interfibrillar substance was influenced in all cases. Fiber bundles subjected to post‐fixation in OsO 4 were particularly denuded of interfibrillar substance. Erythrocytes, also studied for comparison, revealed in all groups a general reduction in size and a certain variability in shape, indicating that they are subject to reversible alterations during the treatment procedures. The drying methods investigated were all acceptable for studies of fibrous, collagenous elements, although the size and morphology of some of the erythrocytes were generally influenced by the procedures.