Premium
The effect of different demineralizing agents on oral mucous membrane
Author(s) -
FEJERSKOV O.
Publication year - 1971
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.1971.tb02007.x
Subject(s) - demineralization , citric acid , chemistry , staining , formic acid , sodium citrate , h&e stain , pathology , dentistry , biochemistry , medicine , enamel paint
– The effect of different demineralizing media (nitric acid, formic acid‐sodium citrate, citric acid‐sodium citrate and EDTA) on oral mucous membrane was studied with special reference to the oral epithelium. All demineralization was carried out at 20°C, except in the case of EDTA, where the effect of treatment at 4°C and 37°C was also studied. The tissue was either single‐embedded in paraffin wax or double‐embedded in celloidin and paraffin wax. Serial sections were stained with hematoxylin‐eosin, van Gieson, and PAS. Nitric acid demineralization resulted in pronounced eosinophilia which obscured cytologic detail and was therefore an unsuitable medium for cytomorphologic investigations. Formic acid‐sodium citrate gave moderate eosinophilia with acceptable cytologic detail. After demineralization in citric acid‐citrate, there was only slight eosinophilia with good staining differentiation between the individual tissue and cell components, which could therefore be clearly distinguished. Tissue demineralized in EDTA at 4°C, 20°C, and 37°C had perfectly preserved cytologic detail, but at 20°C and 37°C there was dissociation of the epithelial cells except in the basal cell layer. EDTA demineralization at 4°C was thus the best procedure when it was desired to preserve the epithelium in situ.