Premium
Glycosaminoglycan‐like components in oral calculus, parotid saliva, and dental plaque
Author(s) -
PAUNIO KEIJO U.,
PAUNIO ILKKA K.
Publication year - 1970
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.1970.tb02096.x
Subject(s) - saliva , cetylpyridinium chloride , calculus (dental) , papain , glycosaminoglycan , chemistry , dental plaque , dentistry , biochemistry , medicine , enzyme , pulmonary surfactant
— A comparative investigation was performed on glycosaminoglycan‐like fractions obtained from calculus, parotid saliva, and dental plaque. The calculus (sub‐gingival and supragingival) was obtained from extracted teeth. Human parotid saliva was collected with an acrylic cup and the dental plaque was gathered from different individuals. The samples were hydrolyzed with papain and the glycosaminoglycan‐like compounds were precipitated with the conventional methods. The analyses were made from the material precipitated with cetylpyridinium chloride (CPC). The results showed that the CPC‐precipitate of the plaque material contained less uronic acids, sulphate. hexoses. sialic acid, and proteins than did calculus and saliva. The results revealed further that the CPC‐precipitate derived from calculus contained phosphate and some calcium. Both saliva and plaque were devoid of these components. The plaque sample lacked the fractions existing in pherograms of both calculus preparation and preparation of parotid saliva (alcian'blue positive). A part of the fractions in the pherograms of calculus and saliva could be stained with lissamine green.