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The correlations between alteration of p16 gene and clinicopathological factors and prognosis in squamous cell carcinomas of the buccal mucosa
Author(s) -
Dong Yuying,
Wang Jie,
Dong Fusheng,
Wang Xu,
Zhang Yinghuai
Publication year - 2012
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.2012.01132.x
Subject(s) - methylation , buccal administration , carcinoma , proportional hazards model , biology , malignancy , pathology , cancer , gene mutation , survival analysis , epidermoid carcinoma , dna methylation , buccal swab , cancer research , medicine , oncology , gene , mutation , microbiology and biotechnology , gene expression , bioinformatics , genetics
J Oral Pathol Med (2012) 41 : 463–469 Objective: To evaluate relationships between the alteration of p16 gene and the clinical status and prognosis of the patients with squamous cell carcinoma of the buccal mucosa. Methods: Thirty buccal cancers were included in the analysis. Deletion analysis was performed by PCR. Point mutation analysis was used by PCR‐SSCP and direct sequencing. Methylation‐specific PCR methods were adopted for the evaluation of p16 methylation. The correlation between alteration of p16 gene and clinicopathological factors buccal cancer was evaluated by Fisher’s exact test. Kaplan–Meier and Cox regression were used to investigate the relationship between p16 alteration and survival time. Results: The frequency of p16 alteration was 63.3% in buccal carcinomas. P16 deletion was associated significantly with tumor size ( P = 0.01). P16 point mutation was associated significantly with differentiation ( P = 0.006). P16 methylation was associated significantly with nodes metastasis ( P = 0.027). The overall survival rate of 30 buccal carcinomas was 53.3%. The Log‐rank test ( P = 0.021) and univariate Cox regression analysis ( P = 0.030) revealed that p16 methylation was significantly associated with the overall survival rate. Multivariate analysis showed that p16 deletion, p16 mutation, and p16 methylation were not statistically significant. Conclusions: The alterations of p16 gene may play a major role in malignancy and development and metastases of buccal carcinoma and may be an excellent marker of aggressive clinical behavior. P16 methylation has a prognostic value in buccal carcinoma but not an independent prognosis factor. P16 point mutation and p16 deletion have not prognostic significance in buccal carcinoma.