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Induction of Sca‐1 in the duct cells of the mouse submandibular gland by obstruction of the main excretory duct
Author(s) -
Purwanti Nunuk,
Tsuji Daisuke,
Azlina Ahmad,
Karabasil Mileva Ratko,
Javkhlan Purevjav,
Hasegawa Takahiro,
Yao Chenjuan,
Akamatsu Tetsuya,
Itoh Kohji,
Hosoi Kazuo
Publication year - 2011
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.2011.01011.x
Subject(s) - submandibular gland , excretory system , duct (anatomy) , salivary gland , immunohistochemistry , ligation , pathology , population , biology , blot , medicine , endocrinology , microbiology and biotechnology , gene , biochemistry , environmental health
J Oral Pathol Med (2011) 40 : 651–658 The effect of ligation of the main excretory duct (MED) of the mouse submandibular gland (SMG) on the expression of Sca‐1, a stem cell antigen, was examined by Western blotting and immunohistochemistry. By Western blotting, the expression of Sca‐1 with a molecular weight of 18 kDa was identified in the normal gland. At 1 day post‐ligation, the expression level of Sca‐1 was strongly increased in the experimental gland and weakly in the contralateral gland, and such expression in both glands decreased at 6 days. By immunohistochemistry, Sca‐1 was detected weakly in the apical membrane of excretory duct (ED) cells of the SMG under the normal condition. By duct ligation, Sca‐1 became expressed strongly in most cells of the two major duct systems, i.e., the striated duct (SD) and granular convoluted tubules (GCT), but was not detected in the acinar (Ac) cells. By fluorescence‐activated cell sorter (FACS) analysis, the number of side population (SP) cells in this gland was found to be increased by ligation. These results imply that Sca‐1‐positive cells may have a role in the duct cell proliferation in the regeneration step elicited by MED ligation‐induced injury.