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Endothelial cells of oral pyogenic granulomas express eNOS and CD105/endoglin: an immunohistochemical study
Author(s) -
Vassilopoulos Spyridon I.,
Tosios Konstantinos I.,
Panis Vassilios G.,
Vrotsos John A.
Publication year - 2011
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.2010.00969.x
Subject(s) - endoglin , enos , immunohistochemistry , pathology , medicine , pyogenic granuloma , lesion , nitric oxide , nitric oxide synthase , biology , cd34 , genetics , stem cell
J Oral Pathol Med (2011) 40 : 345–351 Background: The pyogenic granuloma (PG) is a common localized hyperplastic lesion of the oral cavity. The purpose of the present study was to investigate the immunohistochemical expression of endothelial nitric oxide synthases (eNOS) and CD105/endoglin in oral PGs, to evaluate their involvement in the angiogenetic pathways of the lesion. Materials and Methods: Ninety‐three PGs were included in the study. Sixteen tumors were further sub‐classified as pregnancy tumors (PT) and seventeen as pyogenic granulomas with fibrosis (PGFM). Immunohistochemical expression of eNOS and CD105/endoglin was quantified by computerized image analysis with a semi‐automated system. Percentage of staining and number of objects (positive vessels) were recorded for each case. Results: Intense eNOS expression was seen in 92 of 93 lesions. A statistically significant association was found between eNOS percentage of staining/eNOS positive vascular spaces (objects) and age of the patients (9% increase per decade of life). Approximately 40% less eNOS positive objects were recorded in PGFM compared with PGs. Intense membranous CD105/endoglin expression was seen in all cases. The percentage of CD105/endoglin staining was statistically increased in PGs compared with PT. Approximately 40% less CD105/endoglin objects were found in PGFM compared with PGs; 56% more CD105/endoglin objects were found in tongue lesions, compared with gingival lesions. There was no statistically significant correlation considering percentage of staining and number of objects between CD105/endoglin and eNOS. Conclusions: It is suggested that eNOS and CD105/endoglin are involved in the angiogenetic pathways of PG.