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Cancer stem cell immunophenotypes in oral squamous cell carcinoma
Author(s) -
Oliveira Lucinei R.,
OliveiraCosta João P.,
Araujo Iana M.,
Soave Danilo F.,
Zanetti Juliana S.,
Soares Fernando A.,
Zucoloto Sérgio,
RibeiroSilva Alfredo
Publication year - 2011
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.2010.00967.x
Subject(s) - immunostaining , cd44 , immunophenotyping , cd24 , pathology , immunohistochemistry , medicine , cancer stem cell , cancer , univariate analysis , tissue microarray , basal cell , oncology , cell , antigen , biology , multivariate analysis , immunology , genetics
J Oral Pathol Med (2011) 40 : 135–142 Background: The presence of cancer stem cell (CSC) antigens can be evidenced in some human tumors by phenotypic analysis through immunostaining. This study aims to identify a putative CSC immunophenotype in oral squamous cell carcinoma (OSCC) and determine its influence on prognosis. Methods: The following data were retrieved from 157 patents: age, gender, primary anatomic site, smoking and alcohol intake, recurrence, metastases, histologic classification, treatment, disease‐free survival (DFS), and overall survival (OS). An immunohistochemical study for CD44 and CD24 was performed in a tissue microarray of 157 paraffin blocks of OSCCs. Results: In univariate analysis, the immunostaining pattern showed significant influences in relation to OS for alcohol intake and treatment, as well as for the CD44 + and CD44 − /CD24 − immunophenotypes. The multivariate test confirmed these associations. Conclusions: Based on our results, the CD44 immunostaining and the absence of immunoexpression of these two investigated markers can be used in combination with other clinicopathologic information to improve the assessment of prognosis in OSCC.