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Salivary shedding of HHV‐8 in people infected or not by human immunodeficiency virus 1
Author(s) -
de França Talita Ribeiro T.,
de Araújo Rachel Alcoforado,
Ribeiro Camila Maria B.,
Leao Jair Carneiro
Publication year - 2011
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.2010.00959.x
Subject(s) - saliva , virology , virus , immunology , biology , viral shedding , polymerase chain reaction , dna , kaposi's sarcoma , viral disease , human herpesvirus , medicine , genetics , gene
J Oral Pathol Med (2011) 40 : 97–102 Background: Human herpesvirus 8 (HHV‐8), the main agent involved in the etiopathogenesis of Kaposi’s sarcoma (KS) is primarily transmitted through sexual contact. The potential of saliva as a source of HHV‐8 transmission remains unclear. The purpose of this work was to determine the frequency of HHV‐8 detection in saliva of HIV‐infected individuals and their family contacts. Methods: The study group comprised 210 individuals. Group 1: 35 HIV‐infected patients; group 2: 35 non‐HIV individuals; group 3: two siblings for each patient from group 1; group 4: two siblings for each individual from group 2. Each participant had non‐stimulated whole saliva collected and DNA was extracted. HHV‐8‐DNA amplification from ORF‐26 was performed using a nested PCR protocol. Results: HHV‐8 DNA was detected in saliva from 14/35 (40%) HIV‐infected individuals and 4/35 (11.4%) non‐HIV‐infected individuals (OR = 5.16, CI [1.49–17.88], P = 0.006). It was also possible to amplify HHV‐8 DNA in 11/70 (15.7%) relatives of HIV‐infected participants and 4/70 (5.71%) relatives of non‐HIV‐infected individuals( P = 0.041). Among the 14 group 1 patients with HHV‐8 DNA detected in saliva, eight (57.1%) had a household member in whom HHV‐8 DNA was also amplified (OR = 8, CI [1.58–40.29] P = 0.007). Conclusions: HHV‐8 DNA is frequently found in saliva. HIV‐infected individuals showed a higher frequency of detection of HHV‐8 than healthy controls. HHV‐8 DNA was significantly amplified in saliva of household members of HIV/HHV‐8 co‐infected individuals.