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Polymorphisms in the apoptosis‐associated genes FAS and FASL and risk of oral cancer and malignant potential of oral premalignant lesions in a Taiwanese population
Author(s) -
Wang LiHsuan,
Ting ShuoChun,
Chen ChungHo,
Tsai ChiCheng,
Lung Oliver,
Liu TaChih,
Lee ChiaWen,
Wang YenYun,
Tsai ChinLing,
Lin YingChu
Publication year - 2010
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.2009.00873.x
Subject(s) - oral submucous fibrosis , fas ligand , medicine , logistic regression , haplotype , oncology , cancer , immunology , gastroenterology , genotype , pathology , biology , apoptosis , gene , genetics , programmed cell death
J Oral Pathol Med (2010) 39: 155–161 Background: Our aim was to measure the relationship of FAS (−1377G>A and −670A>G), FASL (−844C>T) gene variants and risk of oral cancer. Methods: Polymerase chain reaction‐restriction fragment length polymorphism (PCR‐RFLP) analysis was used to determine the FAS and FASL polymorphisms in 294 oral squamous cell carcinoma (OSCC), 53 oral submucous fibrosis (OSF), and 84 oral leukoplakia (OL) patients, as well as in 333 healthy controls. A standardized questionnaire was applied to collect demographic data, and potential confounding factors. JMP statistical software was used to analyze the association. Results: FAS and FASL polymorphisms were not correlated with OSCC development or the malignant potential of OL by simple and multivariate logistic regression. However, a two‐ to fourfold difference in the risks of betel quid chewing, alcohol consumption, and smoking on OSCC development were observed between participants with different FAS polymorphisms. FAS polymorphisms were significantly correlated with the malignant potential of OSF. Multivariate logistic regression analysis indicated that FAS A −1377 ‐G −670 vs. G −1377 ‐A −670 haplotype (OR = 2.26, 95% CI = 1.16–4.41) was correlated with the malignant potential of OSF. Conclusions: We suggest that FAS and FASL polymorphisms are not significantly correlated with OSCC development or malignant potential of OL. The impact of substance usage on OSCC development could be differentiated by FAS polymorphisms. FAS A −1377 ‐G −670 haplotype may play a role in the malignant potential of OSF.