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Class II β‐tubulin is a novel marker for human tonsillar M cells and follicular dendritic cells
Author(s) -
Lee JuHan,
Kong SuKang,
Wu ZhengSheng,
Wu Qiang,
Choe Jongseon,
Cho Hyun Yee,
Ha Seung Yeon,
Kee Sun Ho,
Kim YoungSik
Publication year - 2010
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.2009.00865.x
Subject(s) - immunostaining , pathology , biology , tonsil , follicular dendritic cells , palatine tonsil , clusterin , tubulin , immunohistochemistry , microbiology and biotechnology , immunology , medicine , t cell , immune system , microtubule , antigen presenting cell , apoptosis , biochemistry
J Oral Pathol Med (2010) 39 : 533–539 Objective: Membranous (M) cell of the human palatine tonsil is an antigen entry site for mucosal infection, but its location is obscure in histological sections. Recently, a microarray analysis has demonstrated that clusterin, annexin A5, CD44, MMP14, and β‐tubulin are candidate genes of M cell marker in mice. Among these genes, we here describe class II β‐tubulin as a new marker for human tonsillar M cells and follicular dendritic cells (FDCs), and present its usefulness for diagnosis of angioimmunoblastic T‐cell lymphomas (AILTs). Materials and methods: Immunohistochemistry and Western blotting for class II β‐tubulin were performed using 81 cases of lymphoid, gastrointestinal and thyroid tissues, and an FDC cell line, respectively. Double immunostaining with clusterin and class II β‐tubulin were carried out. Results: Class II β‐tubulin localized the M cells and FDCs in the palatine tonsils (10/10, 100%) and adenoids (10/10, 100%). It was colocalized with clusterin in the palatine tonsils. However, class II β‐tubulin staining did not identify intestinal M cells in the intestines. Immunoblot analysis revealed that class II β‐tubulin expression was upregulated in HK cells, a normal FDC cell line. Class II β‐tubulin immunostaining highlighted hyperplastic FDC meshworks in all AILTs (14/14, 100%). Conclusion: Class II β‐tubulin is a specific histochemical marker for human tonsillar M cells and FDCs. Thus, class II β‐tubulin immunostaining may be useful to identify tonsillar M cells and to diagnose FDC proliferative lesions such as AILT.