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Stromal laminin chain distribution in normal, hyperplastic and malignant oral mucosa: relation to myofibroblast occurrence and vessel formation
Author(s) -
Franz Marcus,
Wolheim Anke,
Richter Petra,
Umbreit Claudia,
Dahse Regine,
Driemel Oliver,
Hyckel Peter,
Virtanen Ismo,
Kosmehl Hartwig,
Berndt Alexander
Publication year - 2010
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.2009.00840.x
Subject(s) - stromal cell , laminin , myofibroblast , pathology , basement membrane , immunohistochemistry , stroma , cd31 , biology , integrin , chemistry , cancer research , microbiology and biotechnology , cell , medicine , extracellular matrix , biochemistry , fibrosis
J Oral Pathol Med (2010) 39 : 290–298 Background: The contribution of stromal laminin chain expression to malignant potential, tumour stroma reorganization and vessel formation in oral squamous cell carcinoma (OSCC) is not fully understood. Therefore, the expression of the laminin chains α2, α3, α4, α5 and γ2 in the stromal compartment/vascular structures in OSCC was analysed. Methods: Frozen tissue of OSCC (9× G1, 24× G2, 8× G3) and normal (2×)/hyperplastic (11×) oral mucosa was subjected to laminin chain and α‐smooth muscle actin (ASMA) immunohistochemistry. Results were correlated to tumour grade. The relation of laminin chain positive vessels to total vessel number was assessed by immunofluorescence double labelling with CD31. Results: Stromal laminin α2 chain significantly decreases and α3, α4, α5 and γ2 chains and also ASMA significantly increase with rising grade. The amount of stromal α3, α4 and γ2 chains significantly increased with rising ASMA positivity. There is a significant decrease in α3 chain positive vessels with neoplastic transformation. Conclusions: Mediated by myofibroblasts, OSCC development is associated with a stromal up‐regulation of laminin isoforms possibly contributing to a migration promoting microenvironment. A vascular basement membrane reorganization concerning α3 and γ2 chain laminins during tumour angioneogenesis is suggested.