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Use of quantum dots to detect human papillomavirus in oral squamous cell carcinoma
Author(s) -
Xue J.,
Chen H.,
Fan M.,
Zhu F.,
Diao L.,
Chen X.,
Fan L.,
Li P.,
Xia D.
Publication year - 2009
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.2009.00799.x
Subject(s) - tissue microarray , in situ hybridization , basal cell , human papillomavirus , pathology , epithelium , cell , koilocyte , cancer , medicine , cancer research , oncology , biology , immunohistochemistry , cervical cancer , gene , gene expression , cervical intraepithelial neoplasia , biochemistry , genetics
Objective: To examine the association of oral squamous cell carcinoma with human papillomavirus (HPV) using quantum dots (QD) in situ hybridization (ISH). Methods: Expression of HPV16/18 was analyzed in a representative collection of 21 oral squamous cell carcinomas by tissue microarrays. The presence of HPV16/18 high risk was detected by applying QDISH which is compared with conventional ISH. Results: Seven cases out of 21 (33.3%) were positive for QDISH while 1 out of 21 (4.8%) was positive for ISH, although all of HPV DNA were localized in the nuclei in the spinous and basal cell layer of the epithelium. The difference between these two methods was significant ( P < 0.05). Conclusions: These results demonstrate that the QD might be an efficient method for determination of HPV infection and HPV‐associated oral squamous cell carcinoma.