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FGF‐2‐loaded collagen scaffolds attract cells and blood vessels in rat oral mucosa
Author(s) -
Jansen Richard G.,
Van Kuppevelt Toin H.,
Daamen Willeke F.,
KuijpersJagtman Anne M.,
Von den Hoff Johannes W.
Publication year - 2009
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.2009.00763.x
Subject(s) - myofibroblast , h&e stain , fibroblast , immunohistochemistry , hard palate , medicine , pathology , fibroblast growth factor , blood vessel , wound healing , oral mucosa , anatomy , chemistry , surgery , fibrosis , biochemistry , receptor , in vitro
Background: Wound contraction and scar formation after cleft palate repair impair the growth of the maxilla. The implantation of a growth factor‐loaded scaffold might solve these problems. Methods: The tissue response to fibroblast growth factor (FGF)‐2 loaded collagen scaffolds was evaluated after implantation in the palate of rats. Scaffolds, with and without FGF‐2, were implanted submucoperiosteally in the palate of 25 rats and evaluated after up to 16 weeks. On hematoxylin and eosin (H&E)‐stained sections, the cell density and the number of giant cells within the scaffolds were quantified. Infiltration of inflammatory cells, myofibroblasts, and the number of blood vessels were quantified after immunohistochemistry. Results: The cell density was significantly higher in the FGF‐2 group up to 4 weeks after implantation (102% at 2 weeks, P < 0.001). The number of blood vessels was also significantly higher in the FGF‐2 group at 1 and 2 weeks (316% at 1 week, P = 0.003), but the myofibroblast score was lower (100% at 2 weeks, P = 0.008). A comparable mild and rapidly subsiding inflammatory response and foreign body reaction were found in both groups. Conclusion: FGF‐2‐loaded scaffolds displayed a faster influx of host cells, an increased rate of vascularization, and a reduced differentiation of myofibroblasts. These scaffolds might therefore be highly suitable for intra‐oral reconstructions, such as cleft palate repair.