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Targeted drug delivery system for oral cancer therapy using sonoporation
Author(s) -
Maeda Hironobu,
Tominaga Kazuhiro,
Iwanaga Kenjiro,
Nagao Fuminori,
Habu Manabu,
Tsujisawa Toshiyuki,
Seta Yuji,
Toyoshima Kuniaki,
Fukuda Jinichi,
Nishihara Tatsuji
Publication year - 2009
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.2009.00759.x
Subject(s) - sonoporation , bleomycin , microbubbles , cancer research , medicine , antibody , drug delivery , epidermal growth factor receptor , cancer cell , cancer , pathology , pharmacology , chemotherapy , immunology , chemistry , ultrasound , organic chemistry , radiology
Ultrasound‐mediated destruction of microbubbles has been proposed as an innovative non‐invasive drug delivery system for cancer therapy. We developed a specific drug delivery system for squamous cell carcinoma that uses sonoporation with the anti‐epidermal growth factor receptor (EGFR) antibody. Administration of a low dose of bleomycin (BLM) by sonoporation with the anti‐EGFR antibody produced a marked growth inhibition of Ca9‐22 cells in vitro . In addition, scanning electron microscopic analysis revealed apparent surface deformation of Ca9‐22 cells treated with sonoporation in the presence of the antibody. Interestingly, the population of apoptotic cells was remarkably increased when a low dose of BLM was delivered using sonoporation with the Fab fragment of the anti‐EGFR antibody. These findings indicate that sonoporation with the Fab fragment makes it possible to administer drugs into cells more efficiently and specifically, suggesting a novel application for chemotherapy and gene therapy treatments for oral squamous cell carcinoma.