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Toll‐like receptor activity in Recurrent Aphthous Ulceration
Author(s) -
Borra Ricardo Carneiro,
De Mesquita Barros Fabiana,
De Andrade Lotufo Mônica,
Villanova Fabiolla Elizabeth,
Andrade Priscila Maria
Publication year - 2009
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.2008.00743.x
Subject(s) - medicine , dermatology , toll like receptor , receptor , pathology , innate immune system
Background:  Toll‐like receptors (TLR) are membrane proteins that recognize conserved molecules derived from bacterial, virus, fungal or host tissues. Activation of TLRs causes the production of cytokines that mediate inflammatory responses and drive T helper (Th) 1 and 2 cell development. As an exaggerated Th1 immune response is supposed to be involved in pathogenesis of Recurrent Aphthous Ulceration (RAU), we suggest that RAU patients may have an imbalance in TLR pathways. Methods:  To study the function of TLR activation ex vivo , peripheral blood mononuclear cells (PBMCs) from RAU patients ( n  = 17) and controls ( n  = 17) were exposed to TLR2 [lipoteichoic acid (LTA), heat‐killed Listeria monocytogenes (HKLM) and PamC3CSK4], TLR3 [Poly(I:C)], TLR4 [lipopolysaccharide (LPS)], TLR5 (flagellin) and TLR7 (imiquimod) ligands, and the time course of supernatant tumor necrosis factor‐α (TNF‐α) levels was quantified by enzyme‐linked immunosorbent assay. In addition, serological and salivary TNF‐α and soluble CD14 levels were quantified. The TNF‐α produced by PBMCs in contact with each TLR ligand and autologous serum or saliva at the same time was also investigated. The data were analyzed by statistical multivariate tests. Results:  The control group had a higher response to LTA, whereas RAU had a higher response to HKLM. LTA and LPS interfered with the salivary stimulation of the RAU PBMC and HKLM with the stimulation of the control. Autologous serum was capable of inhibiting TLR2 responsiveness to LTA and enhancing LPS stimulation. Salivary and serological levels of sCD14 and TNF‐α were not significantly different. Conclusion:  Recurrent Aphthous Ulceration patients have an anomalous activity of the TLR2 pathway that probably influences the stimulation of an abnormal Th1 immune response.

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