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Methylation of P16 , P21 , P27 , RB1 and P53 genes in odontogenic keratocysts
Author(s) -
Moreira Paula Rocha,
Guimarães Mariana Moreira,
Guimarães André Luiz Sena,
Diniz Marina Gonçalves,
Gomes Carolina Cavaliéri,
Brito João Artur Ricieri,
Gomez Ricardo Santiago
Publication year - 2009
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.2008.00718.x
Subject(s) - methylation , keratocyst , odontogenic , epigenetics , gene , clinical significance , dna methylation , biology , pathology , cancer research , medicine , gene expression , genetics
Background: Odontogenic keratocyst (OKC) is a benign neoplasm with an aggressive clinical behavior and a high recurrence rate. Although epigenetic alterations have been reported in different tumors, these events were not investigated in OKC yet. The aim of this study was to investigate the presence of methylation in P16, P21, P27, P53 and RB1 genes in OKC tumors. Methods: Methylation‐specific polymerase chain reaction (MSP) was used to evaluate the presence of methylation in 10 samples of OKCs, 10 samples of dental follicles and six samples of normal mucosa. Results: The methylation status of the P16 gene was similar among the three groups. In P21 gene, 30% of OKCs were methylated while no methylation could be detected in the other groups. High frequency of P27 methylation (90%) was observed in dental follicles, however, some OKC lesions (10%) and normal mucosa samples (33%) were also methylated. Concerning the RB1 gene, positive results were detected only in dental follicles (40%). No positive result was observed considering P53 gene. Conclusions: Our data show methylation of the promoter of P21 gene in OKCs. In addition, methylation of the P27 and RB1 genes are commonly found in dental follicles. Further studies are necessary to determine the functional relevance of these alterations.