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Fine deletion analysis of 1p36 chromosomal region in oral squamous cell carcinomas
Author(s) -
Lefeuvre Mathieu,
Gunduz Mehmet,
Nagatsuka Hitoshi,
Gunduz Esra,
Al Sheikh Ali Mahmoud,
Beder Levent,
Fukushima Kunihiro,
Yamanaka Noboru,
Shimizu Kenji,
Nagai Noriyuki
Publication year - 2009
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.2008.00666.x
Subject(s) - loss of heterozygosity , carcinogenesis , biology , locus (genetics) , cancer research , microsatellite instability , microsatellite , basal cell , gene , tumor suppressor gene , cancer , chromosomal region , suppressor , genetics , pathology , allele , medicine
Background: Squamous cell carcinoma is the most common cancer type of the oral cavity and approximately 50% of the patients succumb to the disease. Unfortunately, few are known about the molecular mechanisms involving in the formation of oral squamous cell carcinoma (OSCC). Recently, it has been reported that 1p36 chromosomal region is deleted in various cancer types and is suspected to harbor various tumor suppressor genes (TSGs). However, limited studies exist on genetics alteration on 1p36 in OSCC and the responsible TSG remained unidentified. Methods: To investigate area susceptible to harbor TSG(s) involved in OSCC on 1p36 region, paired normal and tumor tissues of 27 patients with diagnosis of OSCC have been analyzed for loss of heterozygosity (LOH) using nine microsatellite markers based on recent gene mapping. Results: LOH was found at least in one locus in 85% of the cases (23 of 27). Interestingly, microsatellite instability was also found in 7% (two of 27) of the cases analyzed. The higher LOH frequencies were found with the markers D1S243 (25%), D1S468 (22%), D1S450 (25%), D1S228 (38%), D1S199 (28%), and D1S1676 (23%). Conclusions: Three preferentially deleted regions have been identified in OSCC: region 1 (D1S468‐D1S243), region 2 (D1S450‐D1S228), and region 3 (D1S199‐D1S1676). Multiple candidate TSGs, such as RIZ1, p73, UBE4B, Rap1GAP, EPHB2, and RUNX3, are located in these three areas. The data obtained in this study can be used for further functional analysis of these genes involved in OSCC carcinogenesis.