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Increased number of CD25 + FoxP3 + regulatory T cells in oral squamous cell carcinomas detected by chromogenic immunohistochemical double staining
Author(s) -
Schwarz Stephan,
Butz Martin,
Morsczeck Christian,
Reichert Torsten E.,
Driemel Oliver
Publication year - 2008
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.2008.00641.x
Subject(s) - foxp3 , il 2 receptor , immunohistochemistry , cd8 , tumor infiltrating lymphocytes , regulatory t cell , pathology , cancer research , biology , immune system , t cell , immunology , medicine
Background: The role of tumor‐infiltrating regulatory T cells (Treg) compromising antitumor effects of immune cells in oral squamous cell carcinoma (OSCC) is largely unknown. Purpose: The presence of CD25 + FoxP3 + Treg as well as of CD3 + FoxP3 + and of CD8 + FoxP3 + tumor‐infiltrating lymphocytes (TIL) was verified in OSCC and compared with non‐cancerous lymphoepithelial tissue. Method: Three double stainings (CD3/FoxP3, CD8/FoxP3 and CD25/FoxP3) were performed on tissue sections of 15 OSCC and compared with 15 human tonsils. Results: OSCC biopsy samples provide evidence for a strong infiltration of TIL, in particular, naturally occurring CD25 + FoxP3 + Treg. Whereas a comparison of OSCC and control tissue did not show significant changes in the number of CD3 + FoxP3 + TIL and of CD8 + FoxP3 + TIL, a significantly higher frequency of CD25 + FoxP3 + TIL (Treg) could be observed in OSCC ( P < 0.001, two‐sided t ‐test). Given the small number of specimens, a significant correlation with tumor stage could not be verified. Conclusion: Chromogenic double staining of CD4/FoxP3 is a promising tool for the detection of Treg in paraffin‐embedded tissue of OSCC.