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DNA ploidy analysis in salivary gland tumours by image cytometry
Author(s) -
Vargas P. A.,
TorresRendon A.,
Speight P. M.
Publication year - 2007
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.2007.00551.x
Subject(s) - carcinoma ex pleomorphic adenoma , mucoepidermoid carcinoma , pathology , pleomorphic adenoma , salivary gland , adenoid cystic carcinoma , acinic cell carcinoma , biology , carcinoma , adenocarcinoma , feulgen stain , cytometry , adenoma , cancer , flow cytometry , medicine , staining , microbiology and biotechnology , genetics
Aim: To determine whether DNA ploidy by image cytometry is a good diagnostic tool to distinguish benign and malignant salivary gland tumours. Methods: A total of 62 salivary gland tumours were studied. Cases were histologically diagnosed [haematoxylin and eosin (H&E)]. According to the World Health Organization (WHO) classification, there were 14 mucoepidermoid carcinomas (MEC), 11 adenoid cystic carcinomas (ACC), 10 pleomorphic adenomas (PA), 10 carcinoma ex PA (CEPA), 9 acinic cell carcinomas (ACCa), 3 polymorphous low‐grade adenocarcinomas (PLGA), 2 papillary cystadenocarcinomas (PC), 1 myoepithelial carcinoma (MC), 1 undifferentiated carcinoma (UC) and 1 mucinous adenocarcinoma (MA). Paraffin sections (40 μm) were micro‐dissected to isolate tumour areas; cell nuclei were extracted and Feulgen‐stained cytospin monolayers were analysed using a DNA image cytometry system. For each case, DNA index (DI) was calculated relative to internal controls (lymphocytes; DI = 1.0). Cases were categorized as diploid or aneuploid and the proportion of cells over 5c was also calculated. Results: Fifty‐three of 62 salivary gland tumours were uniformly diploid. Only nine cases were aneuploid: five CEPA, one low‐grade MEC, one PC, one UC and one MA. Conclusions: The vast majority of salivary gland tumours were diploid. High‐grade malignancies may be aneuploid, and ploidy may be useful to identify malignant change in atypical PA. Further, larger studies are needed to confirm our results and to further evaluate the usefulness of the technique in high‐grade lesions.