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An orthotopic floor‐of‐mouth model for locoregional growth and spread of human squamous cell carcinoma
Author(s) -
Henson B.,
Li F.,
Coatney D. D.,
Carey T. E.,
Mitra R. S.,
Kirkwood K. L.,
D’Silva N. J.
Publication year - 2007
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.2007.00549.x
Subject(s) - metastasis , floor of mouth , cancer research , pathology , head and neck squamous cell carcinoma , basal cell , medicine , cell culture , head and neck cancer , bioluminescence imaging , carcinoma , cancer , biology , luciferase , transfection , tongue , genetics
The molecular investigation of head and neck cancer targets requires the utilization and optimization of established animal models to characterize the effects of gene transcription and protein expression on invasion and metastasis. Floor‐of‐the‐mouth murine models have been developed to study tumor growth, invasion, and metastasis of murine squamous cell carcinoma (SCC) cells in immunocompetent mice and invasion and metastasis of human SCC cells in nude mice. However, there are tumor cell lines that do not produce tumors in mice, using standard techniques, thus reducing the utility of the model to study specific genetic or treatment conditions. Furthermore, these techniques require large tumor volumes raising the possibility of airway compromise. In this report, we detail significant modifications to the orthotopic floor‐of‐mouth murine model for human SCC to facilitate predictable growth of a large panel of University of Michigan SCC cell lines. Furthermore, we describe the use of bioluminescence and micro‐computed tomography to monitor tumor growth and bony invasion.