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Microarray analysis of IL‐1β‐stimulated chemokine genes in synovial fibroblasts from human TMJ
Author(s) -
Ogura Naomi,
Akutsu Miwa,
Tobe Makiko,
Sakamaki Hiroyuki,
Abiko Yoshimitsu,
Kondoh Toshirou
Publication year - 2007
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.2007.00515.x
Subject(s) - ccl20 , chemokine , gene expression , gene , microarray analysis techniques , gene expression profiling , pathology , synovial membrane , microbiology and biotechnology , downregulation and upregulation , microarray , interleukin 8 , biology , medicine , immunology , arthritis , inflammation , chemokine receptor , genetics
Background: Interleukin (IL)‐1β is thought to play a key role in several pathologic conditions of the temporomandibular joint (TMJ). Gene expression profile of synovial fibroblasts stimulated with IL‐1β was studied by oligonucleotide microarray analysis to elucidate candidate genes associated with intracapsular pathologic conditions of TMJ. Methods: RNA was isolated from synovial fibroblasts from five patients after IL‐1β treatment. Gene expression profiling was performed with a GeneChip. Changes in gene expression were determined by comparing IL‐1β‐treated cells with untreated cells. Results: A total of 121 genes showed a greater than threefold difference in average intensity between untreated and IL‐1β‐treated synovial fibroblasts in five experiments. Five chemokines were among the 10 most upregulated genes, and the most upregulated gene was CCL20. The 121 IL‐1β‐responsive genes included 12 chemokines whose mRNA levels were confirmed by real‐time PCR. Conclusion: These data should provided useful information about the pathologic conditions of TMJ, especially in support of diagnosis and therapeutic approaches to TMJ.