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Collagen XVIII modulation is altered during progression of oral dysplasia and carcinoma
Author(s) -
Väänänen Anu,
Ylipalosaari Merja,
Parikka Mataleena,
Kainulainen Tiina,
Rehn Marko,
Heljasvaara Ritva,
Tjäderhane Leo,
Salo Tuula
Publication year - 2007
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.2006.00498.x
Subject(s) - in situ hybridization , epithelial dysplasia , dysplasia , pathology , basement membrane , immunohistochemistry , carcinoma in situ , carcinoma , epithelium , fluorescence in situ hybridization , messenger rna , medicine , biology , gene , biochemistry , chromosome
Background: Collagen XVIII is a ubiquitous basement membrane (BM) component and a precursor of endostatin. Methods: Using immunohistochemistry and in situ hybridization, we studied the expression and localization of collagen XVIII in different stages of normal oral wound healing, epithelial dysplasia and squamous cell carcinoma (SCC). Results: In mild epithelial dysplasias collagen XVIII appeared as a continuous signal in the BM, whereas in severe epithelial dysplasias and in the invasive areas of oral SCCs collagen XVIII was absent. In situ hybridization showed that collagen XVIII mRNA expression did not decrease in severe dysplasia or oral carcinoma samples when compared with the mild dysplasias. Conclusions: The results indicate that the absence of collagen XVIII protein in severe oral dysplasias is related to the processing of the protein rather than to changes in mRNA expression.