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Establishment and characterization of a spindle cell squamous carcinoma cell line
Author(s) -
Kudo Yasusei,
Ogawa Ikuko,
Kitagawa Masae,
Kitajima Shojiro,
Samadarani Siriwardena B. S. M.,
Aobara Noriko,
Matsuda Chika,
Miyauchi Mutsumi,
Takata Takashi
Publication year - 2006
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.2006.00446.x
Subject(s) - vimentin , cell culture , biology , epithelial–mesenchymal transition , cytokeratin , cell , pathology , immunohistochemistry , carcinosarcoma , cell growth , cancer research , carcinoma , cancer , immunology , medicine , metastasis , genetics
Background: Spindle cell squamous carcinoma (SCSC) is a rare and peculiar biphasic malignant neoplasm that occurs mainly in the upper aerodigestive tract. It consists of sarcomatoid proliferation of pleomorphic spindle‐shaped cells and squamous cell carcinoma. Methods: Here, we established a SCSC cell line from a tumour arisen in gingiva. We characterized the feature of a SCSC cell line by immunohistochemistry. To know the biological feature, we examined the cell growth, invasiveness and epithelial‐mesenchymal transition markers of a SCSC cell line in comparison with oral squamous cell carcinoma (OSCC) cell lines. Results: By immunohistochemical analyses, the primary tumour expressed cytokeratin and vimentin, indicating carcinosarcoma‐like characters. This tumour also showed overexpression of p53 protein. Cultured SCSC cells resulted in bypass of crisis and maintenance over passage 100. The established SCSC cell line was spindle‐shaped and showed identical immunohistochemical characters to those of primary tumour cells. Similar to the primary tumour, the cell line showed p53 overexpression and had p53 mutation at codon 132: AAG (lys)→AAT (asp). The SCSC cell line grew slower than two other OSCC cell lines (MSCC‐1 and HSC‐2), whereas SCSC cells had remarkable invasiveness in comparison with these cell lines. Moreover, SCSC cells expressed wnt‐5a and vimentin mRNA at high levels, but did not express E‐cadherin mRNA. This expression pattern of the markers was similar to that of mesenchymal cells, not of epithelial cells. Conclusion: In the present study, we newly established a SCSC cell line with strong invasiveness. This is the first report on the establishment of SCSC cell line. The SCSC cell line can be a useful cell model for the study to know the cytodifferentiation and nature of SCSC.