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Immunohistochemical analysis of the biological potential of odontogenic keratocysts
Author(s) -
Kolář Zdeněk,
Geierová Marie,
Bouchal Jan,
Pazdera Jindřich,
Zbořil Vítězslav,
Tvrdý Peter
Publication year - 2006
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.2006.00382.x
Subject(s) - nevoid basal cell carcinoma syndrome , radicular cyst , immunohistochemistry , pathology , keratocyst , odontogenic , dentigerous cyst , biology , basal (medicine) , cell cycle , basal cell nevus syndrome , basal cell carcinoma , medicine , cell , basal cell , genetics , insulin , endocrinology
Background: The aim of this study was to analyse the usefulness of detecting important apoptosis and proliferation markers in assessing the biological potential of odontogenic keratocysts (OKC) and thus selecting the optimal diagnostic algorithm for these lesions. Methods: Indirect immunohistochemistry and relevant statistical methods were used for analysis of formalin‐fixed and paraffin‐embedded samples from 98 patients. Results: Nevoid basal cell carcinoma syndrome (NBCCS) keratocysts were characterized by higher expression of Bcl‐2, p27 Kip1 and c‐erbB‐2 as well as by lower proliferative activity measured by Ki‐67 in basal cell epithelium and by a lower inflammatory response in comparison with sporadic keratocysts. Dentigerous, radicular and non‐specified odontogenic cysts differed from both NBCCS and sporadic keratocysts in a wide spectrum of apoptosis and/or cell cycle‐related protein expressions, higher proliferation in the basal cell layer, and vice versa, lower proliferation in the suprabasal cell layer. Conclusions: The NBCCS keratocysts have a different immunophenotype from sporadic keratocysts and both types are distinguishable from dentigerous, radicular and non‐specified odontogenic cysts. These findings confirm the separate biological potential of these lesions and the results of the immunohistochemical analysis have diagnostic and prognostic implications.