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Relationship between TNF‐ α and TUNEL‐positive chondrocytes in antigen‐induced arthritis of the rabbit temporomandibular joint
Author(s) -
Hirota Yuriko,
Habu Manabu,
Tominaga Kazuhiro,
Sukedai Miho,
Matsukawa Akihiro,
Nishihara Tatsuji,
Fukuda Jinichi
Publication year - 2006
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.2006.00367.x
Subject(s) - tunel assay , arthritis , tumor necrosis factor alpha , cartilage , medicine , apoptosis , chondrocyte , temporomandibular joint , dna fragmentation , necrosis , terminal deoxynucleotidyl transferase , pathology , immunohistochemistry , immunology , anatomy , biology , programmed cell death , biochemistry
Background: Terminal deoxynucleotidyl transferase‐mediated deoxyuridine triphosphate biotin nick end‐labeling (TUNEL) staining is a widely accepted method for the detection of DNA fragmentation in nuclei of apoptotic cells. Tumor necrosis factor (TNF)‐ α is closely associated with changes in condylar cartilage and modulates apoptosis in various tissues including cartilage. The aim of this study was to investigate the relationship between apoptotic chondrocytes and TNF‐ α in a rabbit model of arthritis. Method: Unilateral temporomandibular joint (TMJ) arthritis was induced in 20 adult New Zealand White rabbits. From 1 day to 6 weeks after the induction of arthritis, immunohistochemical analysis for TNF‐ α and TUNEL was performed. Results: In condylar cartilage, TNF‐ α ‐positive cells and TUNEL‐positive cells were localized together. TNF‐ α ‐positive chondrocytes seemed to precede TUNEL‐positive cells. Conclusions: The results of the present study suggest that TNF‐ α may be involved in apoptosis and/or apoptotic necrosis of chondrocytes as TMJ arthritis progresses from the acute to chronic stage.