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Immunohistochemical co‐localization of lymphatics and blood vessels in oral squamous cell carcinomas
Author(s) -
Xuan Ming,
Fang YiRu,
Wato Masahiro,
Hata Shintaro,
Tanaka Akio
Publication year - 2005
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.2005.00316.x
Subject(s) - lymphatic system , pathology , lymphatic vessel , immunohistochemistry , cd34 , blood vessel , medicine , lymphatic endothelium , biology , cancer , metastasis , stem cell , genetics
Background: Differentiating lymphatic vessels from blood vessels is difficult, partly due to the lack of a specific method for identifying lymphatics. A new lymphatic vessel‐reactive antibody, D2‐40 has recently become commercially available. We examined the selectivity of D2‐40 for lymphatics in oral neoplastic lesions for discrimination from blood vessels. Methods: Formalin‐fixed, paraffin‐embedded sections of oral lymphangiomas ( n = 3), oral hemangiomas ( n = 7), and oral squamous cell carcinomas (OSCC, n = 46) were double immunostained with D2‐40 and anti‐CD34 monoclonal antibodies (MoAb) using ENVISION‐polymer technique with 5‐bromo‐4‐chloro‐3‐indoxyl‐phosphate (BCIP)/nitroblue tetrazolium chloride (NBT) and 3,3′‐diaminobenzidine (DAB) as color reagents, respectively. Results: In the oral lymphangiomas and hemangiomas D2‐40 was detected in all lymphatics, while all blood vessels were positive for CD34. In OSCC, number of vessels for lymphatics ( P < 0.01) and for blood vessels in the perineoplastic areas were significantly greater than those in intratumoral areas. Conclusions: These results indicate that lymphatic proliferation might be much more extensive in the peritumoral area than intratumoral.