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Assessment of SS‐A and SS‐B in parotid saliva of patients with Sjögren's syndrome
Author(s) -
Hammi Ann Rose,
AlHashimi Ibtisam H.,
Nunn Martha E.,
Zipp Michele
Publication year - 2005
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.2004.00301.x
Subject(s) - saliva , autoantibody , medicine , serology , gastroenterology , kappa , antibody , immunology , linguistics , philosophy
Background: The purpose of this study was to compare the sensitivity of parotid saliva to that of serum in detecting anti‐SSA/Ro and anti‐SSB/La autoantibodies in patients with Sjögren's syndrome. Methods: Forty patients and 20 controls participated in the study; all patients met the 1993 European Community criteria for the diagnosis of Sjögren's syndrome. Healthy controls were age‐ and sex‐matched individuals with no signs or symptoms of Sjögren's syndrome. Serum and saliva samples were evaluated using AffiniTech SSA/Ro and SSB/La antibodies kits (AffiniTech, Ltd. Bentonville, AR, USA). The results were also compared with serological status of SS‐A and SS‐B as reported by an independent clinical laboratory. Results: Serum was significantly more sensitive than saliva in detecting SSA/Ro and SSB/La antibodies ( P = 0.001). There was high agreement between the results with the AffiniTech kits and the independent laboratory (kappa = 0.80; P < 0.001). However, there was poor agreement between saliva and serum results (kappa = 0.174; P = 0.168). Conclusions: The overall results appear to support that serum analysis is effective method for evaluating the presence of SS‐A and SS‐B autoantibodies.