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The differential expression pattern of BMP‐4 between the dentigerous cyst and the odontogenic keratocyst
Author(s) -
Kim SeongGon,
Yang ByoungEun,
Oh SoHee,
Min SooKee,
Hong SamPyo,
Choi JeYong
Publication year - 2005
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.2004.00285.x
Subject(s) - keratocyst , pathology , staining , mesenchymal stem cell , in situ hybridization , immunohistochemistry , epithelium , dentigerous cyst , mesenchyme , biology , medicine , cyst , gene expression , gene , biochemistry
Background: Bone morphogenic protein‐4 (BMP‐4) is widely expressed in oral cavity and involved in tooth morphogenesis, cellular differentiation and proliferation. The purpose of this study was to compare the difference in expression pattern of BMP‐4 in odontogenic keratocysts (OKC) and dentigerous cysts (DC). Methods: We evaluated 77 cysts, OKC ( n = 34) or DC ( n = 43). The average age of patients with OKC was 29.5 ± 14.4 and that of patients with DC was 36.1 ± 19.4. The male to female ratio was 20:14 for OKC and 27:16 for DC. Ten cases of OKC were recurrences. Expression of BMP‐4 was determined by immunohistochemistry and in situ hybridization. Results: The intensity scales were (−) for invisible or trace staining, (+) for visible staining, and (++) for dense, strong staining. OKCs exhibited the following staining patterns: the epithelium in 15/34 specimens and the mesenchymal cells in 17/34 specimens showed (++) stain. In contrast, the staining pattern of DC was (−) for epithelium in 37/43 specimens. The mesenchymal cells showed (−) degree staining in 30/43 specimens. The difference between the groups studied was significant ( P < 0.001 in epithelium and mesenchymal cells). When recurrent and non‐recurrent OKC were compared BMP‐4 was expressed more intensely in the recurrent cases ( P = 0.036 in epithelium). The difference in BMP‐4 expression in mesenchymal cells was not significant. In situ hybridization demonstrated positive mRNA probes to BMP‐4 were localized in epithelium and mesenchymal cells of OKCs and DCs. Conclusions: BMP‐4 was expressed more intensely in OKC when compared with DC, and was more intensely expressed in recurrent cases.