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Angiogenesis in mucous retention cyst: a human in vivo ‐like model of endothelial cell differentiation in mucous substrate
Author(s) -
Swelam Wael,
IdaYonemochi Hiroko,
Saku Takashi
Publication year - 2005
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.2004.00282.x
Subject(s) - pathology , tenascin , cd34 , angiogenesis , cd31 , vascular endothelial growth factor , biology , endothelial stem cell , cd68 , extracellular matrix , microbiology and biotechnology , stem cell , immunohistochemistry , medicine , cancer research , fibronectin , in vitro , biochemistry , vegf receptors
Background: Mucous retention cysts contain a mucous pool in the lumina, in which pure angiogenic processes are occasionally observed. By using this unique human material, our aim was to understand the in vivo angiogenic process. Methods: Fifteen surgical tissue samples of mucous retention cysts of the lip were examined for expression of vascular endothelial markers and extracellular matrix molecules by immunohistochemistry and in situ hybridization (ISH). Results: Endothelial cells forming new vascular channels showed immunopositivities for CD31, CD34, vascular endothelial growth factor (VEGF), and von Willebrand factor (vWF). These newly formed capillaries were surrounded by tenascin‐positive matrices and further by a dense infiltration of CD68‐positive cells with foamy to epitheloid appearances. Some of these cells were simultaneously positive for CD34, VEGF, and one of its receptors, Flk‐1, and they showed definite mRNA as well as protein signals for tenascin. In addition, these cells often tended to be aligned, which suggested tubule formation. Conclusion: The results suggest that monocyte/macrophage lineage cells are a major source for endothelial cells at least in mucous retention cysts and that tenascin produced by those cells plays an important role in differentiation of endothelial cells.