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Microarray gene expression profiling of cell lines from primary and metastatic tongue squamous cell carcinoma: possible insights from emerging technology
Author(s) -
Vigneswaran Nadarajah,
Wu Jean,
Sacks Peter,
Gilcrease Michael,
Zacharias Wolfgang
Publication year - 2005
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.2004.00258.x
Subject(s) - gene expression profiling , gene , biology , gene expression , metastasis , phenotype , microarray analysis techniques , cell culture , cancer research , cell , microarray , primary tumor , epidermoid carcinoma , pathology , carcinoma , cancer , medicine , genetics
Background: To identify common gene expression patterns among two uniquely matched pairs of primary and metastatic oral squamous cell carcinoma (OSCC) cell lines derived from the same two patient donors. Methods: Two pairs of cell lines derived from the primary tumors and lymph node metastases of the same two patients were used to obtain microarray‐based gene expression profiles. Reverse transcriptase‐polymerase chain reaction and immunohistochemistry were used to confirm observed changes for some of the candidate genes. Results: Approximately 50% of the genes profiled were expressed in all four cell lines. Cluster analysis identified a group of 17 genes whose expression correlated inversely with metastatic progression. Only 10 common genes were differentially expressed in both pairs of primary and metastatic cells. A group of 28 highly expressed genes was common for both metastatic cell lines, among them some of the known metastasis‐related genes such as laminin receptor, thymosin β ‐4 and β ‐10 and metallopanstimulin. Conclusions: Groups of presumed metastasis‐related genes are highly heterogeneous and vary significantly between the two patients. Thus, it is unlikely that the metastatic phenotype of these OSCC cells is acquired by de‐regulation of a single gene or a group of few genes. Most likely, multiple combinations of differentially expressed genes are involved in facilitating metastatic spread of these oral carcinoma cell lines.