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The involvement of K v 3.4 voltage‐gated potassium channel in the growth of an oral squamous cell carcinoma cell line
Author(s) -
Lew TienShen,
Chang CheShoa,
Fang KungPing,
Chen ChianYi,
Chen ChengHsiung,
Lin ShuChun
Publication year - 2004
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.2004.00236.x
Subject(s) - transfection , cell culture , cell growth , potassium channel , microbiology and biotechnology , biology , malignant transformation , cell , carcinoma , cancer research , patch clamp , chemistry , pathology , medicine , endocrinology , receptor , biochemistry , genetics
Background: In our previous study, an A‐type voltage‐gated K + channel, K v 3.4, was found more frequently expressed in oral squamous cell carcinoma (OSCC) when compared with non‐cancerous matched oral tissue. An OSCC cell line, OECM‐1, was found to have moderate level of K v 3.4 expression. Methods: To further elucidate the roles of K v 3.4 for the involvement of neoplastic process, we amplified K v 3.4 coding sequence by reverse transcriptase polymerase chain reaction (RT‐PCR), constructed an expression vector carrying this sequence and then stably transfected into OECM‐1 OSCC cells. Results: We demonstrated the integration and constitutive expression of K v 3.4 in the cell. A unique A‐type current elicited by such expression in OECM‐1 cells was defined by patch clamp analysis. This current pattern can be reversibly blocked by an A‐type K + channel blocker 2 mM 4‐aminopyridine (4‐AP). The acquisition of K v 3.4 activity in OECM‐1 cells bestowed growth advantage. However, in 3T3 cell, transfected K v 3.4 caused only limited increase of growth without forming transformation foci. Conclusion: The present study established a stable keratinocyte system carrying functional K v 3.4 and increase of growth, by which the anti‐K v 3.4 modalities for potential OSCC control can be further investigated.