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Immunohistochemical detection of DNA topoisomerase type II α and Ki‐67 in adenoid cystic carcinoma and pleomorphic adenoma of the salivary gland
Author(s) -
Hirabayashi Susumu
Publication year - 1999
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.1999.tb02011.x
Subject(s) - adenoid cystic carcinoma , ki 67 , immunohistochemistry , topoisomerase , pathology , pleomorphic adenoma , salivary gland , carcinoma , adenoma , adenoid , biology , medicine , dna , genetics
Immunohistochemical detection of cell proliferation‐associated antigens was investigated in 28 cases of adenoid cystic carcinoma (ACC) and 20 cases of pleomorphic adenoma (PA), using antibodies against DNA topoisomerase type II α (topo‐II) (Ki‐S1) and Ki‐67 (MIB‐1). The correlation of staining indices with clinicopathological data, histological features and prognosis was also studied. The topo‐II value was significantly higher in ACC than in PA (P<0.0001), and highest in the solid growth pattern of ACC. In addition, significant relationships were found between topo‐II values and clinical features such as local recurrence, surgical margins, and distant metastases. By log‐rank test, the topo‐II index was also correlated significantly with patient survival (P<0.01). The values of topo‐II index paralleled those of Ki‐67 index in ACC, and a correlation coefficient of 0.97 was obtained. Topo‐II may be considered an additional marker for estimating the proliferating fraction of cells and for predicting the short‐term prognosis for patients with salivary gland tumors.