Premium
Bilirubin pigmentation of human teeth caused by hyperbilirubinemia
Author(s) -
Watanabe Kazufumi,
Shibata Toshiyki,
Kurosawa Takao,
Morisaki Ichijiro,
Kinehara Michitoki,
Igarashi Seiji,
Arisue Makoto
Publication year - 1999
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.1999.tb02010.x
Subject(s) - bilirubin , diazo , chloroform , deciduous teeth , acetic acid , chemistry , dentistry , medicine , chromatography , biochemistry , organic chemistry
This study was conducted to identify bilirubin in deciduous teeth obtained from two patients with a history of severe liver dysfunction. Teeth were histologically analyzed and bilirubin was extracted and quantified spectrophotometrically. Histological analysis revealed a green line in the dentine running parallel to the incremental lines. A chloroform/methanol/acetic acid (30:10:0.5, v/v) extract of the teeth was evaporated and the residue dissolved in chloroform. Absorption spectra were prepared before and after the diazo reaction. The absorption maximum shifted from 450 nm before to 540 nm after the diazo reaction and was higher than that of normal deciduous teeth. These results indicate that the discolouration of teeth in patients with severe liver dysfunction is due to bilirubin deposition.