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Expression of E‐cadherin in oral cancer cell lines and its relationship to invasiveness in SCID mice in vivo
Author(s) -
Hoteiya Toshiaki,
Hayashi Eiji,
Satomura Kazuhito,
Kamata Nobouki,
Nagayama Masaru
Publication year - 1999
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.1999.tb02006.x
Subject(s) - in vivo , cadherin , pathology , cancer , biology , medicine , cell culture , cancer research , cell , genetics
We examined the expression of E‐cadherin in nine oral cancer cell lines. HSC‐4, NA, ZA, HOC927 and Ca9–22 cells strongly expressed E‐cadherin [E‐CD(++) cell line] and HSC‐2 and HSC‐3 cells weakly expressed E‐cadherin [E‐CD(+) cell line]. All the cell lines that expressed E‐cadherin were of cuboidal morphology and formed cobblestone colonies. In contrast, TSU and HOC313 cells had spindle shapes, formed dispersed colonies, and were completely negative for E‐cadherin [E‐CD(‐) cell line]. Moreover, all cell lines that expressed E‐cadherin showed tumorgenicity in SCID mice, but E‐CD(‐) cell lines did not show tumorgenicity. The tumors derived from E‐CD(+) cell lines invaded deeper into the connective tissues than those from E‐CD(++) cell lines. In immunohistochemical analysis, the difference was more marked at the edges of the cancer nests. These results suggest that E‐cadherin expression was relevant to the cell forms and the differential grade of cultured cells and that reduced E‐cadherin in oral cancer may be associated with invasiveness in vivo.