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Effect of epidermal growth factor administration on the development of mouse salivary gland carcinomas
Author(s) -
Tsujimoto Hitoshi,
Yura Yoshiaki,
Yoshioka Yukio,
Kusaka Jun,
Yoshida Hideo,
Sato Mitsunobu
Publication year - 1999
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.1999.tb01991.x
Subject(s) - submandibular gland , dmba , epidermal growth factor , salivary gland , carcinogenesis , pathology , carcinoma , medicine , immunohistochemistry , endocrinology , biology , epidermal growth factor receptor , receptor , cancer
This study investigated whether epidermal growth factor (EGF) administration was capable of modifying salivary gland carcinogenesis. Two groups of mice were given 1 mg of 9, 10‐dimethyl‐1,2‐benzanthracene (DMBA) into the left submandibular gland, and then Group 1 mice received 2 μg of EGF and Group 2 mice received vehicle subcutaneously for 8 weeks. Mice in two other groups, 3 and 4, received either EGF or vehicle alone. Twelve weeks after the start of the experiment, the incidences of submandibular gland carcinomas in Groups 1 and 2 were 39% and 58%, respectively, although this difference was not statistically significant. Duct‐ and cyst‐like structures and carcinomas in the left submandibular glands were weakly stained by anti‐EGF receptor (EGFR) antibody. Immunoblot and reverse transcriptase polymerase chain reaction (RT‐PCR) analysesrevealed the expression of EGFR in the submandibular glands and carcinomas. However, EGFR was undetectable in YT cells that were derived from a submandibular gland undifferentiated carcinoma of a Group 2 mouse. These findings indicate that EGF does not promote tumor induction in mouse salivary gland carcinogenesis. This may be ascribed in part to the low expression level of EGFR in tumor cells.