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Overexpression of bcl‐2 protein inhibits terminal differentiation of oral keratinocytes in vitro
Author(s) -
Harada Hidemitsu,
Mitsuyasu Takeshi,
Seta Yuji,
Maruoka Yuka,
Toyoshima Kuniaki,
Yasumoto Shigeru
Publication year - 1998
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.1998.tb02084.x
Subject(s) - involucrin , keratin , oncogene , cellular differentiation , transfection , keratinocyte , biology , apoptosis , in vitro , microbiology and biotechnology , cell culture , keratin 14 , stratified squamous epithelium , epithelium , gene , cell cycle , genetics , transgene , genetically modified mouse
Harada H, Mitsuyasu T, Seta Y. Maruoka Y, Toyoshima K, Yasumoto S: Overexpression of bcl‐2 protein inhibits terminal differentiation of oral keratinocytes in vitro. J Oral Pathol Med 1998; 27: 11–7. © Munksgaard, 1998. The bcl‐2 proto‐oncogene is a known inhibitor of apoptosis; in normal human stratified squamous epithelium, its expression is restricted to the basal cell layer. To investigate the functional role of bcl‐2 protein in the process of differentiation of oral keratinocytes, bcl‐2 expression vector was transfected into SCC‐25 cells, which normally undergo squamous cell differentiation in vitro while expressing specific differentiation markers, e.g., keratin 10/11 and involucrin. In bcl‐2 transfected SCC‐25 cells, the expression of these differentiation markers was markedly suppressed. The bcl‐2 proto‐oncogene may play a critical role in opposing the commitment to terminal differentiation and apoptosis of oral keratinocytes.