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Expression of regulatory apoptotic proteins in peripheral giant cell granulomas and lesions containing osteoclast‐like giant cells
Author(s) -
Pammer J.,
Mazal P.,
Horvat R.,
Weninger W.,
Hulla H.
Publication year - 1998
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.1998.tb01954.x
Subject(s) - giant cell , peripheral blood mononuclear cell , pathology , biology , multinucleate , giant cell tumor of bone , apoptosis , giant cell tumors , cell , microbiology and biotechnology , in vitro , medicine , biochemistry , genetics
Peripheral giant cell granuloma consists of monononuclear cells and osteoclast‐like giant cells. The proliferative ability of peripheral giant cell granuloma is restricted to the mononuclear cell compartment, whereas multinucleated giant cells lack mitotic activity. Although the proliferative compartment of peripheral giant cell granuloma has been investigated in detail, the expression and distribution of proteins regulating apoptosis is unknown. The present study demonstrates strong expression of bak and bax in the majority of giant cells. In contrast, giant cells show only weak positivity for bcl‐2 and moderate positivity for bcl‐x. Mononuclear cells were negative to weakly positive for bcl‐x. Only scattered mononuclear cells were positive for bak, bax and bcl‐2. The frequency of apoptotic nuclei detected by TUNEL‐staining compared to regular nuclei was 18 times higher in giant cells than in mononuclear cells. In summary, our findings support the presumption that giant cells of bone and soft tissue tumors are reactive cell forms and not of neoplastic origin.