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Establishment of ameloblastoma cell line, AM‐1
Author(s) -
Harada Hidemitsu,
Mitsuyasu Takeshi,
Nakamura Norifumi,
Higuchi Yoshinori,
Toyoshima Kuniaki,
Taniguchi Akiyoshi,
Yasumoto Shigeru
Publication year - 1998
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.1998.tb01943.x
Subject(s) - ameloblastoma , cell culture , immortalised cell line , biology , pathology , adamantinoma , cell , cell growth , microbiology and biotechnology , cancer research , medicine , anatomy , genetics , maxilla
Ameloblastomas are slowly growing, locally invasive neoplasms with a potentially destructive behaviour. The molecular mechanisms that regulate the cell growth and invasion of ameloblastoma cells are unknown. Because ameloblastoma cells placed in culture have a very limited lifespan, the establishment of immortalized clones of ameloblastoma cells would aid its study. We produced an immortalized ameloblastoma cell line (AM‐1) using human papillomavirus type‐16. This cell line maintains epithelial cell morphology and expresses cytokeratins K8, K14, K18, K19. Furthermore, bcl‐2 protein, which prevents apoptosis, is expressed. We investigated the behaviour of these cells on a collagen matrix in vitro. These cells grew in a monolayer over foci of collagen degradation and could invade the collagen gel at such sites. Since the behavior of cell line AM‐1 mimics the behavior of ameloblastoma in vivo , it may be a valuable model for the study of these neoplasms.