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Dynamic distribution of basic fibroblast growth factor during epulis formation: an immunohistochemical study in an enhanced healing process of the gingiva
Author(s) -
Murata Masashi,
Hara Kohji,
Saku Takashi
Publication year - 1997
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.1997.tb01228.x
Subject(s) - basic fibroblast growth factor , granulation tissue , pathology , wound healing , mast cell , immunohistochemistry , angiogenesis , neovascularization , fibrosis , fibroblast growth factor , fibroblast , growth factor , biology , medicine , immunology , cancer research , cell culture , genetics , receptor
Basic fibroblast growth factor (bFGF) is thought to play an important role in wound healing. However, its histological localization, both in normal and pathological conditions in the oral mucosa, has not been well documented. We have studied the immunolocalization of bFGF in normal gingiva and gingivaf epulis specimens corresponding to different organizing stages. In normal gingiva. bFGF was detected in subpopulations of macrophages. mast cells and most endothelial cells in the lamina propna. Granulation tissue in epulides was histopathologically classified into six organizing stages. In stages 1 and 2. a small number of bFGF‐positive macrophages was seen at the periphery of ulcer bases. In stages 3 and 4. histologically characterized by prominent capillary proliferation, large numbers of bFGF‐positive macrophages and mast celis were located within granulation tissue. A positive reaction for bFGF was also found in some endothelia! cells and in myxoedematous stroma that was rich in heparan sulfate proteoglycan. In stages 5 and 6, when fibrosis was accelerated. bFGF‐positive macrophages and mast cells decreased in number and were localized only at the periphery of the fibrous tissue. These findings suggest that maximum amounts of bFGF are synthesized and released from some macrophages and mast cells into the extracellular matrix during neovascularization of granulation tissue.