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Expression and mutations of p53 in salivary gland tumours
Author(s) -
Kärjä Vesa J.,
Syrjänen Kari J.,
Kurvinen AnnaKaisa,
Syrjänen Stina M.
Publication year - 1997
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.1997.tb01227.x
Subject(s) - mucoepidermoid carcinoma , salivary gland , pathology , exon , immunohistochemistry , p53 expression , single strand conformation polymorphism , adenoid cystic carcinoma , p53 protein , adenoid , biology , carcinoma , tumor suppressor gene , gene , medicine , carcinogenesis , genetics
A series of 219 salivary gland tumours (103 carcinomas and 116 benign tumours) were analysed for p53 protein expression using immunohistochemistry, and for mutations in p53 gene using non‐radioactive single strand conformation polymorphism (SSCP). p53 expression was present in 36% (42/116) of the benign tumours and in 54% (56/103) of the carcinomas. The highest prevalence of p53 expression was found in adenoid cystic carcinomas (69%). followed by mucoepidermoid carcinomas (67%). Of the benign tumours, pleomorphic adenomas showed the highest prevalence of p53 positivity (41%). In malignant tumours, expression of p53 bore no correlation to local recurrence, metastatic disease or survival of the patients. Exons 5 through 9 were analysed and four mutations were found in 20 cases of p53‐immunopositive tumours and two in 20 p53‐negative tumours. Each of the exons 5.6 and 8/9 had two mutations, whereas no mutations were detected in exon 7.