Premium
Human herpesvirus‐6 (HHV‐6) DNA and virus‐encoded antigen in oral lesions
Author(s) -
Yadav Manmohan,
Arivananthan Meena,
Chandrashekran Anil,
Tan Bee Siew,
Hashim Bin Yaacob
Publication year - 1997
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.1997.tb00238.x
Subject(s) - immunohistochemistry , antigen , biology , in situ hybridization , digoxigenin , virus , pathology , polymerase chain reaction , leukoplakia , oral lichen planus , monoclonal antibody , antibody , oral mucosa , virology , microbiology and biotechnology , cancer , medicine , immunology , gene , messenger rna , biochemistry , genetics
Archival oral tissues comprising 51 squamous cell carcinomas, 18 non‐malignant lesions and 7 normal mucosa samples were investigated for human herpesvirus‐6 (HHV‐6)‐encoded antigens and HHV‐6 DNA. The virus‐specific antigens were detected by an immunohistochemical method using monoclonal antibodies. Two further techniques used for HHV‐6 DNA detection included the polymerase chain reaction (PCR) with virus‐specific primers and in situ hybridization using digoxigenin‐labelled oligonucleotides specific for HHV‐6A and HHV‐6B genotypes. A high proportion (79‐80%) of the squamous cell carcinomas were positive for HHV‐6 with the various detection methods. In cases of lichen planus and leukoplakia a high prevalence rate (67‐100%) was noted with in situ hybridization and immunohistochemical techniques but a lower proportion (22–33%) was detected with the PCR method. All 7 normal tissues tested were negative for HHV‐6. The HHV‐6 variant B was found in 60% of the oral carcinoma tissues analysed. The study demonstrates the frequent presence of HHV‐6 in neoplastic and non‐malignant lesions of the oral cavity. While the role of HHV‐6 in oral mucosal tissues remains to be determined, the in vitro tumorigenic potential of the virus suggests a possible role in the etiopathogenesis of oral lesions.