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Single cell gel electrophoresis on peripheral blood leukocytes of patients with oral squamous cell carcinoma
Author(s) -
Rao G. Venkateswara,
Kumar G. S.,
Ahuja Y. R.
Publication year - 1997
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.1997.tb00234.x
Subject(s) - grading (engineering) , pathology , cell , dna damage , basal cell , comet assay , gel electrophoresis , carcinoma , malignancy , epidermoid carcinoma , biology , medicine , dna , microbiology and biotechnology , ecology , genetics
The single cell gel electrophoresis (SCGE) assay, also known as the comet assay, is a cytogenetic technique for measuring and analyzing DNA single stranded breaks (SSB) and/or alkali labile sites within individual cells. Peripheral blood leukocytes of 22 oral squamous cell carcinoma (OSCC) patients were subjected to SCGE and the DNA damage levels (SSB) were quantified with respect to clinical staging and histopathologic grading. Highly statistically significant differences in DNA damage levels were found between normal subjects and patients with OSCC of the same age group. DNA damage levels were altered in all clinical stages and histopathological grades of oral squamous cell carcinoma. The differences were generally significant between all the clinical stages of OSCC. while in histopathologic grading the results were significant only between grades 1 and 111. The results support the concept of a systemic host response in malignancy.